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Mitochondria, Volume 65 (Methods in Cell Biology) | |||||
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Autor: Pon, Liza A. AnotaceThis title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs. The book is organized in a convenient bench-top format. It is full of step-by-step "how to" methods with sample results, interpretations, and pitfalls. There is a unique set of appendices that include gene catalogs, mtDNA maps, and reagents for probing respiratory chain function. Finally, there are applications of state-of-the art microarray and gene chip technologies. Key Features* Modern, proven methods that impact on all aspects of mitochondrial structure, function, and biogenesis* Theoretical basis for all methods; not only how they work, but why they work* Contributions from leaders in the field, including Guiseppi Attardi, Ron Butow, and Walter NeupertTopics Covered Include:* Isolation of mitochondria from commonly used cells and tissues* Assays for mitochondrial activities, including respiration, ATP production, permeability, protein import, and interactions with the cytoskeleton* Biochemical and optical methods for studying protein-protein interactions in mitochondria* Approaches to studying mitochondrial replication, transcription, and translation* Transmitochondrial technologies* Methods in microassay data analysis Dostupné zdroje
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