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Real-Time PCR: An Essential Guide (Horizonbioscience) | |||||
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Rok: 2004 AnotaceThe Horizon Scientific Press titles focus on high-level microbiology and molecular biology topics. Written by internationally renowned and highly respected leaders in the field, titles in this series comprise of review manuals, practical manuals, and reference texts for research scientists, bioscience professionals and graduate students.Real-time PCR is a powerful and rapid technique for nucleic acid amplification. The accumulation of specific products in a reaction is monitored continuously during cycling. This is usually achieved by monitoring changes in fluorescence within the CPR tube. Real-Time PCR presents a comprehensive guide to the most appropriate and up-to-date technologies and applications and provides an overview of the theory of this important technique. Written by recognized experts in the field, this timely and authoritative volume is an essential requirement for all laboratories using PCR. Topics covered include: real-time PCR instruments and probe chemistries, set-up, controls and validation, quantitative real-time PCR, analysis of mRNA expression, mutation detection, NASBA, application in clinical microbiology and diagnosis of infection. Dostupné zdroje
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