Analysis of Free Radicals in Biological Systems

Rok: 1995
ISBN: 9783764351373
OKCZID: 110584318

Anotace

The main aim of the book is to provide a comprehensive survey on recent methodological aspects of the measurement of damage within cellular targets, information which may be used as an indicator of oxidative stress. In the introductory chapters, emphasis is placed on the chemical properties of reactive oxygen species and their role in the induction of cellular modifications together with their links to various diseases. The central part of the book is devoted to the description of selected methods aimed at monitoring the production of free radicals in cellular systems. In addition, several assays are provided to assess the chemical damage induced by reactive oxygen species in critical cellular-targets in vitro and in humans in vivo. Thus both practical aspects and general considerations, including discussions on the applications and limitations of the assays, are critically reviewed. One of the major features of the book is the description of new experimental methods. These include the measurement of oxydized DNA bases and nucleosides, new techniques for the determination of LDL oxidation using spin-trap agents, and the use of salicylate as an indicator of oxidative stress. In addition, more classical though significantly improved techniques devoted to the measurement of hydroperoxides and aldehydes are described. The book will serve a large scientific community including biologists, chemists, and clinicians working on the chemical and biological effects of oxidative stress. It may also be of interest to investigators in the fields of drug, cosmetic and new food research.

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